| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA3.1/Myc-His A , pcDNA3.1/Myc-HisA |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5494 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | C-His, C-Myc |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Neomycin (G418) |
| 备注: | |
| 产品目录号: | V855-20 |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pcDNA3.1/Myc-His A质粒图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格：¥1920
- 货 号：BiovectorpcDNA3.1/Myc-His A
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
pcDNA3.1/Myc-HisA载体基本信息
pcDNA3.1/Myc-HisA载体质粒图谱和多克隆位点信息
pcDNA3.1/Myc-HisA载体简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
pcDNA3.1/Myc-HisA载体序列
LOCUS pcDNA3.1(+)/myc-His A 5494 bp DNA circular SYN
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..5494
/invitrogen="126"
/vntifkey="98"
CDS 1042..1059
/invitrogen="1010000"
/vntifkey="4"
/label=6xHis
CDS complement(4498..5358)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1088..1312
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1082..1099)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5359..5457)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
CDS 997..1026
/invitrogen="1090000"
/vntifkey="4"
/label=c-myc\epitope
rep_origin 1358..1786
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
misc_feature 902..999
/invitrogen="1490000"
/vntifkey="21"
/label=MCS
CDS 2197..2991
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(3680..4353)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 1791..2161
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3167..3297
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
BASE COUNT 1273 a 1427 c 1400 g 1394 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
961 gcacagtggc ggccgctcga gtctagaggg cccttcgaac aaaaactcat ctcagaagag
1021 gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
1081 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
1141 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
1201 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
1261 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
1321 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
1381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
1441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
1501 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
1561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
1621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
1681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
1741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
1801 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
1861 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
1921 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
1981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2041 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
2101 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
2161 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
2221 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
2281 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
2341 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
2401 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
2461 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
2521 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
2581 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
2641 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
2701 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
2761 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
2821 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
2881 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
2941 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
3001 tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
3061 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
3121 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
3181 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
3241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
3301 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
3361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
3421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
3481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
3541 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
3601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
3661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
3721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
3781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
3841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
3901 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
3961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
4021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
4081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
4141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
4201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
4321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
4381 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
4441 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
4501 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
4561 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
4621 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
4681 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
4741 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
4801 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
4861 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
4921 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
4981 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
5041 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
5101 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
5161 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
5221 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
5281 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
5341 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
5401 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
5461 gcgcacattt ccccgaaaag tgccacctga cgtc您正在向 biovector.net 发送关于产品 pcDNA3.1/Myc-His A质粒图谱序列抗性价格报价Biovector NTCC Inc.现货 的询问
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