出品公司: | Invitrigen |
---|---|
载体名称: | pEF6/V5-His TOPO |
质粒类型: | 哺乳细胞表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | EF-1α |
克隆方法: | TOPO-TA |
载体大小: | 5840 bp |
5' 测序引物及序列: | T7 Forward:TAATACGACTCACTATAGGG |
3' 测序引物及序列: | -- |
载体标签: | His Tag (6x) ; V5 Epitope Tag |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | Blasticidin |
备注: | Cleavage:EK (Enterokinase) Recognition Site ;高表达水平。 |
产品目录号: | K9610-20 |
稳定性: | 瞬表达 |
组成型: | 组成型表达 |
病毒/非病毒: | 非病毒 |
pEF6/V5-His TOPO TA质粒图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥1920
- 货 号:BiovectorpEF6/V5-His TOPO TA
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pEF6/V5-His TOPO载体基本信息
pEF6/V5-His TOPO载体质粒图谱和多克隆位点信息
pEF6/V5-His TOPO载体简介
The pEF6/V5-His TOPO TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO Cloning. The pEF6/V5-His-TOPO vector includes:Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells The Blasticidin resistance gene for rapid selection of stable mammalian cell lines A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
pEF6/V5-His TOPO载体序列
LOCUS pEF6/V5 His TOPO 5840 bp DNA SYN DEFINITION pEF6/V5 His TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5840 /organism="pEF6/V5 His TOPO" /mol_type="other DNA" promoter complement(28..56) /label="AmpR_promoter" promoter 470..1653 /label="EF1a_promoter" CDS complement(773..1393) /label="ORF frame 2" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 1601..1621 /label="EF1a_fwd_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 1670..1688 /label="T7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(1917..1934) /label="BGH_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 1920..2147 /label="bGH_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2210..2516 /label="f1_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2630..2650) /label="pBABE_3_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2636..2852) /label="SV40_enhancer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 2648..2917 /label="SV40_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2816..2893 /label="SV40_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 2878..2897 /label="SV40pro_F_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 3011..3078 /label="EM7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene 3079..3477 /label="blast" /gene="blast" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 3638..3757 /label="SV40_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3726..3745 /label="EBV_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3801..3819) /label="M13_reverse_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(3818..3840) /label="M13_pUC_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3854..3883) /label="lac_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin complement(4192..4811) /label="pBR322_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene complement(4966..5826) /label="Ampicillin" /gene="Ampicillin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" CDS complement(4966..5826) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 61 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 121 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA 181 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG 241 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT 301 GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA 361 TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA 421 GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGCCTC GTGAGGCTCC 481 GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG 541 GTCGGCAATT GAACCGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC 601 GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC 661 GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGGTAAG TGCCGTGTGT 721 GGTTCCCGCG GGCCTGGCCT CTTTACGGGT TATGGCCCTT GCGTGCCTTG AATTACTTCC 781 ACCTGGCTGC AGTACGTGAT TCTTGATCCC GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT 841 TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG 901 CGCTGGGGCC GCCGCGTGCG AATCTGGTGG CACCTTCGCG CCTGTCTCGC TGCTTTCGAT 961 AAGTCTCTAG CCATTTAAAA TTTTTGATGA CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT 1021 AGTCTTGTAA ATGCGGGCCA AGATCTGCAC ACTGGTATTT CGGTTTTTGG GGCCGCGGGC 1081 GGCGACGGGG CCCGTGCGTC CCAGCGCACA TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG 1141 CCACCGAGAA TCGGACGGGG GTAGTCTCAA GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC 1201 GCGCCGCCGT GTATCGCCCC GCCCTGGGCG GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG 1261 TGAGCGGAAA GATGGCCGCT TCCCGGCCCT GCTGCAGGGA GCTCAAAATG GAGGACGCGG 1321 CGCTCGGGAG AGCGGGCGGG TGAGTCACCC ACACAAAGGA AAAGGGCCTT TCCGTCCTCA 1381 GCCGTCGCTT CATGTGACTC CACGGAGTAC CGGGCGCCGT CCAGGCACCT CGATTAGTTC 1441 TCGAGCTTTT GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT 1501 CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC 1561 TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT 1621 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTA GCTTGGTACT AATACGACTC 1681 ACTATAGGGA GACCCAAGCT GGCTAGGTAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC 1741 AGTGTGGTGG AATTGCCCTT AGGGCAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC 1801 GAGTCTAGAG GGCCCGCGGT TCGAAGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 1861 TTCTACGCGT ACCGGTCATC ATCACCATCA CCATTGAGTT TAAACCCGCT GATCAGCCTC 1921 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 1981 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 2041 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 2101 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA 2161 AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC 2221 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 2281 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 2341 AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 2401 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 2461 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 2521 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG 2581 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT 2641 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGGCAGGC AGAAGTATGC AAAGCATGCA 2701 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 2761 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 2821 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 2881 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 2941 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 3001 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC 3061 AAGGTGAGGA ACTAAACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA 3121 AGAGCAACGG CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA 3181 GCTCTCTCTA GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA 3241 CCTTGTGCAG AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT 3301 TGTATCGTCG CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGTCGA 3361 CAGGTGCTTC TCGATCTGCA TCCTGGGATC AAAGCGATAG TGAAGGACAG TGATGGACAG 3421 CCGACGGCAG TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAAGCA 3481 CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT 3541 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG 3601 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG 3661 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 3721 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC 3781 TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 3841 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 3901 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 3961 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 4021 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 4081 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 4141 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 4201 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 4261 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 4321 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 4381 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 4441 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 4501 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 4561 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 4621 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 4681 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 4741 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 4801 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 4861 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 4921 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 4981 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 5041 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 5101 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 5161 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 5221 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 5281 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 5341 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 5401 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 5461 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 5521 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 5581 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 5641 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 5701 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 5761 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 5821 ACTCATACTC TTCCTTTTTC
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