出品公司: | Invitrigen |
---|---|
载体名称: | pEF4/V5-His C |
质粒类型: | 哺乳细胞表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | EF-1α |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5765 bp |
5' 测序引物及序列: | T7 Forward:TAATACGACTCACTATAGGG |
3' 测序引物及序列: | -- |
载体标签: | His Tag (6x), V5 Epitope Tag |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | Zeocin |
备注: | 易于克隆到其它载体中去;高表达水平。 |
产品目录号: | V941-20 |
稳定性: | 瞬表达 |
组成型: | 组成型表达 |
病毒/非病毒: | 非病毒 |
pEF4/V5-His C质粒图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥4920
- 货 号:BiovectorpEF4/V5-His C
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pEF4/V5-HisC载体基本信息
pEF4/V5-HisC载体质粒图谱和多克隆位点信息
pEF4/V5-HisC载体简介
pEF4/V5-HisC载体序列
LOCUS pEF4/V5 His C 5765 bp DNA SYN DEFINITION pEF4/V5 His C ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5765 /organism="pEF4/V5 His C" /mol_type="other DNA" promoter complement(28..56) /label="AmpR_promoter" promoter 470..1653 /label="EF1a_promoter" CDS complement(773..1393) /label="ORF frame 2" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 1601..1621 /label="EF1a_fwd_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 1670..1688 /label="T7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(1894..1911) /label="BGH_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 1897..2124 /label="bGH_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2187..2493 /label="f1_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2607..2627) /label="pBABE_3_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2613..2829) /label="SV40_enhancer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 2625..2894 /label="SV40_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2793..2870 /label="SV40_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 2855..2874 /label="SV40pro_F_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 2988..3055 /label="EM7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene 3056..3427 /label="bleo" /gene="bleo" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3056..3430 /label="sh_ble" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 3563..3682 /label="SV40_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3651..3670 /label="EBV_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3726..3744) /label="M13_reverse_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(3743..3765) /label="M13_pUC_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3779..3808) /label="lac_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin complement(4117..4736) /label="pBR322_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene complement(4891..5751) /label="Ampicillin" /gene="Ampicillin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" CDS complement(4891..5751) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 61 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 121 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA 181 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG 241 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT 301 GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA 361 TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA 421 GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGCCTC GTGAGGCTCC 481 GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG 541 GTCGGCAATT GAACCGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC 601 GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC 661 GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGGTAAG TGCCGTGTGT 721 GGTTCCCGCG GGCCTGGCCT CTTTACGGGT TATGGCCCTT GCGTGCCTTG AATTACTTCC 781 ACCTGGCTGC AGTACGTGAT TCTTGATCCC GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT 841 TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG 901 CGCTGGGGCC GCCGCGTGCG AATCTGGTGG CACCTTCGCG CCTGTCTCGC TGCTTTCGAT 961 AAGTCTCTAG CCATTTAAAA TTTTTGATGA CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT 1021 AGTCTTGTAA ATGCGGGCCA AGATCTGCAC ACTGGTATTT CGGTTTTTGG GGCCGCGGGC 1081 GGCGACGGGG CCCGTGCGTC CCAGCGCACA TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG 1141 CCACCGAGAA TCGGACGGGG GTAGTCTCAA GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC 1201 GCGCCGCCGT GTATCGCCCC GCCCTGGGCG GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG 1261 TGAGCGGAAA GATGGCCGCT TCCCGGCCCT GCTGCAGGGA GCTCAAAATG GAGGACGCGG 1321 CGCTCGGGAG AGCGGGCGGG TGAGTCACCC ACACAAAGGA AAAGGGCCTT TCCGTCCTCA 1381 GCCGTCGCTT CATGTGACTC CACGGAGTAC CGGGCGCCGT CCAGGCACCT CGATTAGTTC 1441 TCGAGCTTTT GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT 1501 CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC 1561 TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT 1621 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTA GCTTGGTACT AATACGACTC 1681 ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC 1741 AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGGT CACCCATTCG 1801 AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC 1861 ACCATCACCA TTGAGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC 1921 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG 1981 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC 2041 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG 2101 CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG 2161 GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 2221 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 2281 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT 2341 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 2401 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 2461 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 2521 TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 2581 AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC 2641 AGGCTCCCCA GGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT 2701 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT 2761 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG 2821 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT 2881 CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 2941 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA 3001 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 3061 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 3121 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 3181 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 3241 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 3301 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 3361 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 3421 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 3481 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 3541 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 3601 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 3661 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 3721 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 3781 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 3841 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 3901 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 3961 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 4021 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 4081 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 4141 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 4201 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 4261 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 4321 TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 4381 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 4441 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 4501 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 4561 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 4621 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 4681 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 4741 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 4801 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 4861 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 4921 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 4981 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 5041 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 5101 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 5161 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 5221 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 5281 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 5341 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 5401 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 5461 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 5521 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 5581 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 5641 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 5701 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 5761 TTTTC //
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