出品公司: | Invitrigen |
---|---|
载体名称: | pEF4/myc-His A |
质粒类型: | 哺乳细胞表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | EF-1α |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5882 bp |
5' 测序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
3' 测序引物及序列: | -- |
载体标签: | 6XHis, c-Myc Epitope |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | Zeocin |
备注: | 克隆宿主菌株E.coli TOP10F',DH5αF',INVαF';高表达水平。 |
产品目录号: | V942-20 |
稳定性: | -- |
组成型: | 组成型表达 |
病毒/非病毒: | 非病毒 |
pEF4/myc-His A质粒图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥4920
- 货 号:BiovectorpEF4/myc-His A
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pEF4-myc-HisA载体基本信息
pEF4-myc-HisA载体质粒图谱和多克隆位点信息
pEF4-myc-HisA载体简介
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
pEF4-myc-HisA载体序列
LOCUS pEF4/myc-His A 5882 bp DNA SYN DEFINITION pEF4/myc-His A ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5882 /organism="pEF4/myc-His A" /mol_type="other DNA" promoter complement(28..56) /label="AmpR_promoter" promoter 470..1653 /label="EF1a_promoter" CDS complement(773..1393) /label="ORF frame 2" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 1601..1621 /label="EF1a_fwd_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 1670..1688 /label="T7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(1889..1906) /label="BGH_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 1892..2119 /label="bGH_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2182..2488 /label="f1_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2602..2622) /label="pBABE_3_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2608..2824) /label="SV40_enhancer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 2620..2889 /label="SV40_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2788..2865 /label="SV40_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 2850..2869 /label="SV40pro_F_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 2983..3050 /label="EM7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene 3051..3422 /label="bleo" /gene="bleo" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3051..3425 /label="sh_ble" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 3558..3677 /label="SV40_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3646..3665 /label="EBV_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3721..3739) /label="M13_reverse_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(3738..3760) /label="M13_pUC_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3774..3803) /label="lac_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin complement(4112..4731) /label="pBR322_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene complement(4886..5746) /label="Ampicillin" /gene="Ampicillin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" CDS complement(4886..5746) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5788..5816) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 61 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 121 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA 181 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG 241 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT 301 GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA 361 TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA 421 GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGCCTC GTGAGGCTCC 481 GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG 541 GTCGGCAATT GAACCGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC 601 GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC 661 GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGGTAAG TGCCGTGTGT 721 GGTTCCCGCG GGCCTGGCCT CTTTACGGGT TATGGCCCTT GCGTGCCTTG AATTACTTCC 781 ACCTGGCTGC AGTACGTGAT TCTTGATCCC GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT 841 TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG 901 CGCTGGGGCC GCCGCGTGCG AATCTGGTGG CACCTTCGCG CCTGTCTCGC TGCTTTCGAT 961 AAGTCTCTAG CCATTTAAAA TTTTTGATGA CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT 1021 AGTCTTGTAA ATGCGGGCCA AGATCTGCAC ACTGGTATTT CGGTTTTTGG GGCCGCGGGC 1081 GGCGACGGGG CCCGTGCGTC CCAGCGCACA TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG 1141 CCACCGAGAA TCGGACGGGG GTAGTCTCAA GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC 1201 GCGCCGCCGT GTATCGCCCC GCCCTGGGCG GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG 1261 TGAGCGGAAA GATGGCCGCT TCCCGGCCCT GCTGCAGGGA GCTCAAAATG GAGGACGCGG 1321 CGCTCGGGAG AGCGGGCGGG TGAGTCACCC ACACAAAGGA AAAGGGCCTT TCCGTCCTCA 1381 GCCGTCGCTT CATGTGACTC CACGGAGTAC CGGGCGCCGT CCAGGCACCT CGATTAGTTC 1441 TCGAGCTTTT GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT 1501 CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC 1561 TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT 1621 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTA GCTTGGTACT AATACGACTC 1681 ACTATAGGGA GACCCAAGCT GGCTAGGTAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC 1741 AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC 1801 TTCGAACAAA AACTCATCTC AGAAGAGGAT CTGAATATGC ATACCGGTCA TCATCACCAT 1861 CACCATTGAG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT 1921 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT 1981 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG 2041 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG 2101 GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT 2161 CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 2221 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 2281 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA 2341 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 2401 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 2461 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 2521 TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 2581 TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT 2641 CCCCAGGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 2701 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 2761 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 2821 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC 2881 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 2941 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT 3001 CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT 3061 TGACCAGTGC CGTTCCGGTG CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA 3121 CCGACCGGCT CGGGTTCTCC CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG 3181 ACGACGTGAC CCTGTTCATC AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG 3241 CCTGGGTGTG GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA 3301 CGAACTTCCG GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC 3361 GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG 3421 ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 3481 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 3541 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 3601 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 3661 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 3721 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 3781 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 3841 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 3901 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 3961 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 4021 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 4081 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 4141 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 4201 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 4261 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 4321 AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 4381 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 4441 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 4501 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 4561 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 4621 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 4681 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 4741 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 4801 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 4861 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 4921 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 4981 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 5041 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 5101 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 5161 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 5221 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 5281 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 5341 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 5401 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 5461 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 5521 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 5581 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 5641 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 5701 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 5761 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 5821 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 5881 TC
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