出品公司: | Invitrigen |
---|---|
载体名称: | pEF4/His C |
质粒类型: | 哺乳细胞表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | EF-1α |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5773 bp |
5' 测序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
3' 测序引物及序列: | -- |
载体标签: | Xpress Epitope, 6XHis |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | Zeocin |
备注: | 易于克隆到其它载体中去。 |
产品目录号: | V94320 |
稳定性: | -- |
组成型: | 组成型表达 |
病毒/非病毒: | 非病毒 |
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pEF4-His C载体基本信息
pEF4-His C载体质粒图谱和多克隆位点信息
pEF4-His C载体简介
pEF4-His C载体序列
LOCUS pEF4/His C 5773 bp DNA SYN DEFINITION pEF4/His C ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5773 /organism="pEF4/His C" /mol_type="other DNA" promoter complement(28..56) /label="AmpR_promoter" promoter 470..1653 /label="EF1a_promoter" CDS complement(773..1393) /label="ORF frame 1" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 1601..1621 /label="EF1a_fwd_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 1670..1688 /label="T7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 1750..1768 /label="Xpress_fwd_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 1751..1783 /label="T7_leader" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 1787..1810 /label="Xpress_EK" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(1902..1919) /label="BGH_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 1905..2132 /label="bGH_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2195..2501 /label="f1_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2615..2635) /label="pBABE_3_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(2621..2837) /label="SV40_enhancer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 2633..2902 /label="SV40_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin 2801..2878 /label="SV40_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 2863..2882 /label="SV40pro_F_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter 2996..3063 /label="EM7_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene 3064..3435 /label="bleo" /gene="bleo" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3064..3438 /label="sh_ble" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" terminator 3571..3690 /label="SV40_PA_terminator" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3659..3678 /label="EBV_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3734..3752) /label="M13_reverse_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature complement(3751..3773) /label="M13_pUC_rev_primer" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" promoter complement(3787..3816) /label="lac_promoter" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" rep_origin complement(4125..4744) /label="pBR322_origin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" gene complement(4899..5759) /label="Ampicillin" /gene="Ampicillin" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" CDS complement(4899..5759) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 61 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 121 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA 181 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG 241 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT 301 GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA 361 TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA 421 GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGCCTC GTGAGGCTCC 481 GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG 541 GTCGGCAATT GAACCGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC 601 GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC 661 GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGGTAAG TGCCGTGTGT 721 GGTTCCCGCG GGCCTGGCCT CTTTACGGGT TATGGCCCTT GCGTGCCTTG AATTACTTCC 781 ACCTGGCTGC AGTACGTGAT TCTTGATCCC GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT 841 TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG 901 CGCTGGGGCC GCCGCGTGCG AATCTGGTGG CACCTTCGCG CCTGTCTCGC TGCTTTCGAT 961 AAGTCTCTAG CCATTTAAAA TTTTTGATGA CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT 1021 AGTCTTGTAA ATGCGGGCCA AGATCTGCAC ACTGGTATTT CGGTTTTTGG GGCCGCGGGC 1081 GGCGACGGGG CCCGTGCGTC CCAGCGCACA TGTTCGGCGA GGCGGGGCCT GCGAGCGCGG 1141 CCACCGAGAA TCGGACGGGG GTAGTCTCAA GCTGGCCGGC CTGCTCTGGT GCCTGGCCTC 1201 GCGCCGCCGT GTATCGCCCC GCCCTGGGCG GCAAGGCTGG CCCGGTCGGC ACCAGTTGCG 1261 TGAGCGGAAA GATGGCCGCT TCCCGGCCCT GCTGCAGGGA GCTCAAAATG GAGGACGCGG 1321 CGCTCGGGAG AGCGGGCGGG TGAGTCACCC ACACAAAGGA AAAGGGCCTT TCCGTCCTCA 1381 GCCGTCGCTT CATGTGACTC CACGGAGTAC CGGGCGCCGT CCAGGCACCT CGATTAGTTC 1441 TCGAGCTTTT GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT 1501 CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC 1561 TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT 1621 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTA GCTTGGTACT AATACGACTC 1681 ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTTACCATG GGGGGTTCTC ATCATCATCA 1741 TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC TGTACGACGA 1801 TGACGATAAG GTACCAGGAT CCAGTGTGGT GGAATTCTGC AGATATCCAG CACAGTGGCG 1861 GCCGCTCGAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG 1921 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC 1981 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA 2041 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG 2101 CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG 2161 CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT 2221 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT 2281 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC 2341 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA 2401 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC 2461 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT 2521 CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT 2581 GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 2641 AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 2701 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 2761 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 2821 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 2881 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 2941 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT 3001 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA 3061 ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG 3121 GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC 3181 GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG 3241 GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG 3301 GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG 3361 CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG 3421 GCCGAGGAGC AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA 3481 AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT 3541 CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA 3601 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT 3661 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG 3721 AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT 3781 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC 3841 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC 3901 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT 3961 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA 4021 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 4081 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 4141 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 4201 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 4261 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 4321 GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 4381 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 4441 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 4501 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 4561 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 4621 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 4681 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 4741 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 4801 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 4861 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 4921 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 4981 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 5041 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 5101 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 5161 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC 5221 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA 5281 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 5341 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT 5401 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 5461 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG 5521 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG 5581 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 5641 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA 5701 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 5761 CTCTTCCTTT TTC
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