| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA3.1(-) |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5427 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | / |
| 载体抗性: | 氨苄 |
| 筛选标记: | 新霉素/G418 |
| 备注: | 与其他pcDNA3.1系列载体差异主要是载体抗性; pcDNA3.1(+)/pcDNA3.1(-)载体的差异在于F1复制点的方向不同。 |
| 产品目录号: | V795-20 |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pcDNA3.1(-)质粒图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格：¥1920
- 货 号：BiovectorpcDNA3.1(-)
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
• Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
• Neomycin resistance gene for selection of stable cell lines
• Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.
载体序列
LOCUS pcDNA3.1(-) 5427 bp DNA circular SYN 2-SEP-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..5427
/invitrogen="129"
/vntifkey="98"
CDS complement(4431..5291)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1027..1251
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1021..1038)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5292..5390)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
rep_origin 1297..1725
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
misc_feature 895..1010
/invitrogen="1620000"
/vntifkey="21"
/label=MCS
CDS 2135..2929
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(3616..4286)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 1730..2099
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3103..3233
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
BASE COUNT 1253 a 1414 c 1382 g 1378 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
961 accacactgg actagtggat ccgagctcgg taccaagctt aagtttaaac cgctgatcag
1021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
1081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
1141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
1201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
1261 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
1321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
1381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
1441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
1501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
1561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
1621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
1681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
1741 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
1801 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
1861 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
1921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
1981 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
2041 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
2101 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
2161 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
2221 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
2281 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
2341 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
2401 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
2461 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
2521 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
2581 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
2641 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
2701 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
2761 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
2821 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
2881 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
2941 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
3001 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
3061 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
3121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
3181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
3241 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
3301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
3361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
3421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
3481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
3541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
3601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
3661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
3721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
3781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
3841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
3901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
3961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
4021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
4081 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
4141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
4201 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
4261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
4321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
4381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
4441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
4501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
4561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
4621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
4681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
4741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
4801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
4861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
4921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
4981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
5041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
5101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
5161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
5221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
5281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
5341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
5401 tttccccgaa aagtgccacc tgacgtc
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