出品公司: | Clontech |
---|---|
载体名称: | pDsRED2-ER |
质粒类型: | 哺乳细胞表达载体;荧光蛋白报告系统 |
高拷贝/低拷贝: | -- |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4.7kb |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 卡纳霉素(Kanamycin) |
筛选标记: | 新霉素(Neomycin) |
备注: | -- |
产品目录号: | 6982-1 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
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- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDsRed2-ER 4757 bp DNA circular 8-JAN-2010 COMMENT Created by Clontech Laboratories Inc. COMMENT Cat. No. 632409 COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers misc_feature 1..589 /label=CMV\IE\Promoter misc_feature 597..647 /label=Calreticulin\signal\sequence misc_feature 663..1337 /label=DsRed2 misc_feature 1350..1361 /label=KDEL\sequence misc_feature 1576..1581 /label=SV40\early\polyA\signal misc_feature 1605..1610 /label=SV40\early\polyA\signal misc_feature 1673..2128 /label=f1\origin misc_feature 2190..2218 /label=Bacterial\promoter\for\Kan(R) misc_feature 2302..2531 /label=SV40\early\Promoter/Enhancer misc_feature 2469..2604 /label=SV40\origin misc_feature 2653..3447 /label=Kan(R)/Neo(R) misc_feature 3683..3688 /label=HSV\TK\polyA\signal misc_feature 3696..3701 /label=HSV\TK\polyA\signal misc_feature 4032..4675 /label=pUC\origin BASE COUNT 1123 a 1289 c 1265 g 1080 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgc 601 tgctatccgt gccgttgctg ctcggcctcc tcggcctggc cgtcgccgac cggtcgcaca 661 ccatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg 721 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 781 gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca 841 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc 901 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 961 aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct 1021 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga 1081 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg 1141 agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca 1201 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg 1261 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc 1321 gccaccacct gttcctgaga tcgtacaaga aggacgagct gtaaagatct cgagctcaag 1381 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga 1441 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 1501 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 1561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 1621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta 1681 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 1741 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 1801 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 1861 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 1921 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 1981 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 2041 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 2101 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga 2161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 2221 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc 2281 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 2341 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 2401 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 2461 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 2521 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 2581 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga 2641 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 2701 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 2761 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 2821 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 2881 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 2941 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 3001 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 3061 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 3121 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 3181 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 3241 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 3301 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 3361 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 3421 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 3481 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 3541 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 3601 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg 3661 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt 3721 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac 3781 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg 3841 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt 3901 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 3961 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 4021 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 4081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 4141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 4201 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtatca ccgcctacat 4261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4681 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4741 gtattaccgc catgcat
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