出品公司: | Clontech |
---|---|
载体名称: | pDsRED2-Mito |
质粒类型: | 哺乳细胞表达载体;荧光蛋白报告系统 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4715bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 卡纳霉素(Kanamycin) |
筛选标记: | 新霉素(Neomycin) |
备注: | -- |
产品目录号: | 632421 |
稳定性: | 瞬时表达 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
pDsRED2-Mito质粒图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥2920
- 货 号:BiovectorpDsRED2-Mito
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDsRed2-Mito 4715 bp DNA circular 8-JAN-2010 COMMENT Created by Clontech Laboratories Inc. http://www.biovector.net/ COMMENT Cat. No. 632421 COMMENT VNTAUTHORNAME|www.biovector.net| FEATURES Location/Qualifiers misc_feature 1..589 /label=CMV\IE\Promoter misc_feature 597..683 /label=Mitochondrial\targeting\sequence misc_feature 705..1382 /label=DsRed2 misc_feature 1534..1539 /label=SV40\early\polyA\signal misc_feature 1563..1568 /label=SV40\early\polyA\signal misc_feature 1631..2086 /label=f1\origin misc_feature 2148..2176 /label=Bacterial\promoter\for\Kan(R) misc_feature 2260..2489 /label=SV40\early\Promoter/Enhancer misc_feature 2427..2562 /label=SV40\origin misc_feature 2611..3405 /label=Kan(R)/Neo(R) misc_feature 3641..3646 /label=HSV\TK\polyA\signal misc_feature 3654..3659 /label=HSV\TK\polyA\signal misc_feature 3990..4693 /label=pUC\origin BASE COUNT 1105 a 1282 c 1260 g 1068 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt 601 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc 661 cgcgcgccaa gatccattcg ttgggggatc caccggtcgc caccatggcc tcctccgaga 721 acgtcatcac cgagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg 781 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc 841 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc 901 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt 961 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga 1021 ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg 1081 gcgtgaactt cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggcct 1141 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagacccac aaggccctga 1201 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc 1261 ccgtgcagct gcccggctac tactacgtgg acgccaagct ggacatcacc tcccacaacg 1321 aggactacac catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt 1381 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 1441 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 1501 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 1561 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 1621 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 1681 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 1741 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 1801 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 1861 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 1921 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 1981 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 2041 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 2101 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 2161 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct 2221 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 2281 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 2341 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 2401 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2461 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 2521 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc 2581 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 2641 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 2701 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 2761 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 2821 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 2881 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 2941 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 3001 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 3061 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 3121 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 3181 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 3241 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 3301 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 3361 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 3421 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 3481 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 3541 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac 3601 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa 3661 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 3721 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 3781 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 3841 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat 3901 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 3961 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 4021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 4081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 4141 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 4201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 4261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 4321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 4381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 4441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 4501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 4561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 4621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 4681 ctgattctgt ggataaccgt attaccgcca tgcat
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