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pMSCV-TAP逆病毒载体质粒图谱序列抗性

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pMSCV-TAP 
(BioVector-012570)

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Available to Academic and Nonprofits Only

BACKBONE

  • Vector backbone
    pMSCV-TAP
  • Backbone manufacturer
    Modified from Clontechs pMSCV
  • Backbone size (bp)6800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

SEQUENCE INFORMATION

  • Depositor Sequences
    None.
  • Addgene Sequences

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • TAP (N terminal on backbone)

CLONING INFORMATION

RESOURCE INFORMATION

  • A portion of this plasmid was derived from a plasmid made by
    TAP was PCRd from pBSactTAP, a gift from Dr. Matthias Wilm (Heidelberg, Germany)
  • Terms and Licenses

DEPOSITOR COMMENTS

A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5 -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3 and right primer 5 -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3 . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See authors map for more information (Note that the NotI site is not precisely underlined in the authors map).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-TAP was a gift from David Sabatini (Addgene plasmid # 12570)
  • For your References section:

    mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001PubMed 16919458
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