pCMV-Tet3G哺乳细胞表达；四环素调控载体质粒图谱序列抗性价格报价Biovector NTCC Inc.现货

LOCUS       pCMV-Tet3G	7139 bp 	DNA	SYN
DEFINITION  pCMV-Tet3G
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="pCMV-Tet3G"
                     /mol_type="other DNA"
     promoter        90..666
                     /label="CMV_immearly_promoter"
     misc_feature    169..456
                     /label="CAG_enhancer"
     misc_feature    623..643
                     /label="CMV_fwd_primer"
     promoter        624..693
                     /label="CMV_promoter"
     CDS             628..1521
                     /label="ORF frame 1"

     promoter        636..755
                     /label="CMV2_promoter"

     misc_feature    667..686
                     /label="pCEP_fwd_primer"

     misc_feature    669..693
                     /label="LNCX_primer"

     CDS             complement(1227..1682)
                     /label="ORF frame 1"

     terminator      complement(1541..1660)
                     /label="SV40_PA_terminator"

     misc_feature    complement(1555..1574)
                     /label="EBV_rev_primer"

     gene            complement(3144..4004)
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             complement(3144..4004)
                     /label="ORF frame 1"

     promoter        complement(4046..4074)
                     /label="AmpR_promoter"

     terminator      complement(4232..4351)
                     /label="SV40_PA_terminator"

     misc_feature    complement(4246..4265)
                     /label="EBV_rev_primer"

     CDS             complement(4379..4852)
                     /label="ORF frame 2"

     misc_feature    complement(4781..4801)
                     /label="SV40 NLS"

     misc_feature    complement(4781..4795)
                     /label="NLS"

     misc_feature    complement(4982..4997)
                     /label="SV40_int"
            
     CDS             5365..5901
                     /label="ORF frame 1"

     CDS             complement(5416..6210)
                     /label="ORF frame 3"
     

     gene            complement(5419..6198)
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"

     promoter        complement(6299..6348)
                     /label="NEOKAN_promoter"

     promoter        complement(6619..6887)
                     /label="SV40_promoter"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    complement(6639..6658)
                     /label="SV40pro_F_primer"

     rep_origin      complement(6643..6720)
                     /label="SV40_origin"

     misc_feature    6690..6905
                     /label="SV40_enhancer"

     misc_feature    6885..6905
                     /label="pBABE_3_primer"

     misc_feature    complement(6943..6965)
                     /label="pGEX_3_primer"

ORIGIN
    1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt
   61 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
  121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
  181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
  241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
  301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
  361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
  421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
  481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
  541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
  601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
  661 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
  721 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccatgtct
  781 agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg agtcggtatc
  841 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
  901 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
  961 catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
 1021 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
 1081 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
 1141 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
 1201 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
 1261 accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg gcagggagcc
 1321 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
 1381 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
 1441 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
 1501 cttgacatgc tccccgggta actaagtaag gatccagaca tgataagata cattgatgag
 1561 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
 1621 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
 1681 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
 1741 ctctacaaat gtggtatggc tgattatgat cctgcaagcc tcgtcgtcct ggccggacca
 1801 cgctatctgt gcaaggtccc cggccccgga cgcgcgctcc atgagcagag cgcccgccgc
 1861 cgaggcgaag actcgggcgg cgccctgccc gtcccaccag gtcaacaggc ggtaaccggc
 1921 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg
 1981 aagtatccag ctcgaccaag cttggcgaga ttttcaggag ctaaggaagc taaaatggag
 2041 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
 2101 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct gcattaatga
 2161 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
 2221 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
 2281 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
 2341 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
 2401 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
 2461 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
 2521 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
 2581 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
 2641 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
 2701 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
 2761 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
 2821 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
 2881 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
 2941 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
 3001 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
 3061 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
 3121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
 3181 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
 3241 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
 3301 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
 3361 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
 3421 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
 3481 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
 3541 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
 3601 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
 3661 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
 3721 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
 3781 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
 3841 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
 3901 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
 3961 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
 4021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
 4081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
 4141 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
 4201 cgtcttcact cgaggtcgag ggatccagac atgataagat acattgatga gtttggacaa
 4261 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
 4321 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
 4381 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
 4441 tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat tccttattaa
 4501 cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag
 4561 acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc
 4621 tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt
 4681 cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca
 4741 aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg
 4801 gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg
 4861 agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat
 4921 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
 4981 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
 5041 caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc
 5101 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga
 5161 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc
 5221 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct
 5281 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
 5341 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
 5401 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
 5461 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
 5521 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
 5581 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
 5641 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
 5701 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
 5761 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
 5821 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
 5881 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
 5941 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
 6001 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
 6061 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
 6121 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
 6181 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
 6241 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
 6301 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
 6361 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
 6421 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
 6481 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
 6541 tgagtgcttg cggcagcgtg aagctagctt tttgcaaaag cctaggcctc caaaaaagcc
 6601 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
 6661 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
 6721 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
 6781 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
 6841 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
 6901 attccacagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
 6961 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
 7021 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
 7081 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcacct