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载体基本信息
| Promega |
| pSI载体 |
| 哺乳动物细胞表达载体 |
| CMV |
| 高 |
| 多克隆位点，限制性内切酶 |
| 3632 bp |
| SV40 pro-F: 5'-TATTTATGCAGAGGCCGAGG-3' |
| EBV-R: 5'-GTGGTTTGTCCAAACTCATC-3' |
| / |
| 氨苄 |
| / |
/ |
| E1721 |
| 稳表达/ 瞬表达 |
| 组成型 Constitutive |
| 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
PCI和PSI 哺乳动物表达载体，旨在提升哺乳动物细胞中插入的克隆DNA基因的组成型表达。这两个载体之间的差异在于增强子/启动子区域，该区域能够控制插入基因的表达水平。 PSI表达载体包含猿猴病毒40（SV40）增强子和早期启动子区域（early promoter region），而PCI表达载体含有人巨细胞病毒（CMV）的主要及时早期基因增强子/启动子区域（immediate-early gene enhancer/promoter region）。
PSI和PCI载体可用于哺乳细胞的瞬时和稳定基因表达。对于哺乳细胞基因的稳表达，PSI或PCI载体必须共转染一个可筛选的表达载体。
载体序列
LOCUS pSI 3632 bp DNA circular SYN
DEFINITION pSI
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..3632
/organism="pSI"
/mol_type="other DNA"
misc_feature complement(79..99)
/label="pBABE_3_primer"
misc_feature complement(85..300)
/label="SV40_enhancer"
promoter 97..365
/label="SV40_promoter"
rep_origin 264..341
/label="SV40_origin"
misc_feature 326..345
/label="SV40pro_F_primer"
misc_feature 483..615
/label="chim_int"
promoter 660..678
/label="T7_promoter"
terminator complement(743..862)
/label="SV40_PA_terminator"
misc_feature complement(757..776)
/label="EBV_rev_primer"
rep_origin 1065..1371
/label="f1_origin"
misc_feature 1689..1711
/label="pGEX_3_primer"
promoter 1870..1898
/label="AmpR_promoter"
gene 1940..2800
/label="Ampicillin"
/gene="Ampicillin"
CDS 1940..2800
/label="ORF frame 2"
rep_origin 2955..3574
/label="pBR322_origin"
ORIGIN
1 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG
61 GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
121 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT
181 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
241 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
301 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
361 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG
421 ATTCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG TTGGTCGTGA GGCACTGGGC
481 AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA
541 GACAGAGAAG ACTCTTGCGT TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG
601 CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT
661 AATACGACTC ACTATAGGCT AGCCTCGAGA ATTCACGCGT GGTACCTCTA GAGTCGACCC
721 GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT
781 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
841 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
901 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA
961 ATCGATAAGG ATCCGGGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
1021 CAGTTGCGCA GCCTGAATGG CGAATGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
1081 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
1141 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
1201 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
1261 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
1321 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
1381 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
1441 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA
1501 TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
1561 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
1621 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
1681 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG
1741 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
1801 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
1861 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
1921 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
1981 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
2041 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
2101 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
2161 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
2221 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
2281 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
2341 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
2401 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
2461 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
2521 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT
2581 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
2641 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC
2701 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA
2761 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC
2821 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
2881 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
2941 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
3001 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
3061 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
3121 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
3181 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
3241 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
3301 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
3361 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
3421 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
3481 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
3541 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
3601 CTGGCCTTTT GCTCACATGG CTCGACAGAT CT
//
DEFINITION pSI
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..3632
/organism="pSI"
/mol_type="other DNA"
misc_feature complement(79..99)
/label="pBABE_3_primer"
misc_feature complement(85..300)
/label="SV40_enhancer"
promoter 97..365
/label="SV40_promoter"
rep_origin 264..341
/label="SV40_origin"
misc_feature 326..345
/label="SV40pro_F_primer"
misc_feature 483..615
/label="chim_int"
promoter 660..678
/label="T7_promoter"
terminator complement(743..862)
/label="SV40_PA_terminator"
misc_feature complement(757..776)
/label="EBV_rev_primer"
rep_origin 1065..1371
/label="f1_origin"
misc_feature 1689..1711
/label="pGEX_3_primer"
promoter 1870..1898
/label="AmpR_promoter"
gene 1940..2800
/label="Ampicillin"
/gene="Ampicillin"
CDS 1940..2800
/label="ORF frame 2"
rep_origin 2955..3574
/label="pBR322_origin"
ORIGIN
1 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG
61 GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
121 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT
181 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
241 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
301 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
361 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG
421 ATTCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG TTGGTCGTGA GGCACTGGGC
481 AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA
541 GACAGAGAAG ACTCTTGCGT TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG
601 CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT
661 AATACGACTC ACTATAGGCT AGCCTCGAGA ATTCACGCGT GGTACCTCTA GAGTCGACCC
721 GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT
781 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
841 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
901 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA
961 ATCGATAAGG ATCCGGGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
1021 CAGTTGCGCA GCCTGAATGG CGAATGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
1081 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
1141 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
1201 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
1261 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
1321 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
1381 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
1441 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA
1501 TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
1561 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
1621 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
1681 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG
1741 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
1801 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
1861 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
1921 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
1981 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
2041 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
2101 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
2161 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
2221 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
2281 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
2341 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
2401 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
2461 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
2521 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT
2581 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
2641 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC
2701 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA
2761 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC
2821 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
2881 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
2941 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
3001 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
3061 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
3121 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
3181 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
3241 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
3301 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
3361 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
3421 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
3481 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
3541 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
3601 CTGGCCTTTT GCTCACATGG CTCGACAGAT CT
//
载体质粒图谱pdf版和相关资料下载
载体应用举例
pEE12.4
pEE6.4
pcDNA3.1/His A
pCHO1.0
pSV-Beta-Galactosidase
pSV2-DHFR
pmirGLO
pCI-neo
pEYFP-C1
pCMV-Tag3
pIRES2-DsRed-Express
pCMV6-AC-GFP
pcDNA3
pDsRED2-ER
pmCherry-C1
p3xFLAG-KV2.1
pGL3-control
pEGFP-1
pDsRed2-N1
pDsRed2-C1
pEF1/V5-HisA
pEF1/myc-His B
pcDNA4/TO
pcDNA4/V5-His A
pcDNA4/HisMax B
pEF6/myc-His C
pEF4/V5-His A
SuperCos1
pDsRED-Monomer-N1
pmCherry-N1
pDsRed2-1
pAcGFP1-1
pLEGFP-C1
pDsRed-Monomer
pEBFP-N1
pGluc-basic
psiCHECK 2
pRNA-H1.1/Adeno
pRNATin-H1.2/Neo
pRNATin-H1.2/Retro
pRNATin-H1.2/Hygro
pEYFP-N1
pECFP-N1
pECFP-C1
pSecTag2 A
pCMV-Tag 3B
pEGFP-N3
pBudCE4.1
pIRESneo3
pcDNA3.1/Myc-His B
pcDNA3.1/Myc-His C
pcDNA3.1/Myc-His A
pcDNA3.1/His C
pcDNA3.1/His B
pVitro2-neo-mcs
pFLAG-CMV2
pCMV-HA
pCMV-myc
pCAGGS
pSG5
pCMV-Tag2 B
pCMV-Tag 5B
pSFV1
pcDNA3.1(+)/Myc HisB
pUB6/V5 His B
pUB6/V5 His A
pUB6/V5 His C
pRL-TK
pNeoEGFP
c-Flag pcDNA3
pcDNA3.1/Hygro(-)
pcDNA6-Myc/His B
pCMV6-Entry
pCMV6-XL4
p3xFLAG-KV4.2
p3xFLAG-KV1.5
p3xFLAG-CMV-10
pIRES
pIRES-EGFP
pCEP4
pGL3 basic
pcDNA3.1(-)
pEGFP-C3
pGL3-Promoter
pcDNA3.1-EGFP
pcDNA3.1(+)
pGCSi/H1/Neo ShGFP
pDsRED2-Mito
pMIR-Report Luciferase
pIRES2-EGFP
pEGFP-C1
pEGFP-N1
PiggyBac Dual promoter
Super PiggyBac Transposase
pBC1
pDsRED2-Nuc
pDsRED2-Peroxi
pEBFP-C1
pECFP-ER
pECFP-Mito
pEYFP-actin
pEYFP-Golgi
pEYFP-Mem
pEYFP-tub
pGL3-Enhancer
pRL-CMV
pRL-SV40
pOG44
pORF-lacZ
pORF-HSV1tk
pEGFP-Actin
pWHERE
pN3
pEGFP-C2
pEGFP-N2
p3xFlag-CMV-14
pIRES-hrGFP-1a
pcDNA3.1/GS
pcDNA3.1/Zeo(+)
pcDNA3.1/Zeo(-)
pIREShyg3
pIRESpuro3
pGenesil-1
pBK-CMV
pNTAP-B
pVAX1
pIRESneo
pIRESneo2
pSI
pCI
pcDNA3.1/Hygro(+)
pBI-CMV1
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