pET-11c(+)图谱序列抗性价格Biovector质粒库

价　　格：¥0
货　　号：39589853061
产　　地：北京

点击询问我要采购

竭诚为您服务！

BioVector NTCC典型培养物保藏中心: 联系人：Dr.Xu, Biovector NTCC Inc.; 电话：400-800-2947 工作QQ:1843439339 (微信同号); 邮件：Biovector@163.com; 手机：18901268599; 地址：北京; 已注册

详细信息
咨询记录(共0条)

pet11c载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列: 载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

EMD Biosciences(Novagen)

pET11c, pet 11c

大肠杆菌蛋白表达

高

多克隆位点，限制性内切酶

5672bp

5'-TAATACGACTCACTATAGGG-3'

N-T7

Ampicillin

Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.

69438-3

瞬时表达 Transient

组成型 Constitutive

非病毒

pet11c载体质粒图谱和多克隆位点信息

pet11c载体图谱

Feature Name
Start
End

T7_terminator	316	188
T7_Terminal_primer	256	274
T7_leader	358	326
Xpress_fwd_primer	359	341
T7_transl_en_RBS	383	367
lacO	428	401
T7_promoter	446	428
pBRrevBam_primer	549	530
lacI	824	1915
tet (576 - 1190)	1974	2588
ROP	3227	3418
pGEX_3_primer	3434	3412
Ampicillin	5464	4604
AmpR_promoter	5534	5506

ORF
Start
End

ORF frame 1	583	1083
ORF frame 2	956	1915
ORF frame 1	2393	1698
ORF frame 3	1971	2588
ORF frame 2	5464	4604

Enzyme Name
Cut

ClaI	24
HindIII	29
BamHI	319
NheI	350
NdeI	357
XbaI	395
BglII	461
BclI	1197
ApaI	1394
HpaI	1689
EagI	2251
NruI	2286
MscI	2758
PstI	4922
AatII	5599
EcoRI	5670

pet11c载体简介

The pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map. The cloning/expression region of the coding strand transcribed byT7 RNA polymerase is shown below.

pet11c载体序列

1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA
61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT
181 GCGGGATATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC CCCTCAAGAC CCGTTTAGAG
241 GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT
301 CGGGCTTTGT TAGCAGCCGG ATCCGACCCA TTTGCTGTCC ACCAGTCATG CTAGCCATAT
361 GTATATCTCC TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA
421 CAATTCCCCT ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCTCGAT CCTCTACGCC
481 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
541 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
601 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
661 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
721 CAGGAGTCGC ATAAGGGAGA GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT
781 TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC
841 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT
901 GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC
961 GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT
1021 GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT
1081 TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG
1141 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT
1201 CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT
1261 TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA
1321 TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC
1381 GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA
1441 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
1501 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT
1561 GGTTGCCAAC GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG
1621 CGTTGGTGCG GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT
1681 CCCGCCGTTA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG
1741 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT
1801 GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
1861 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
1921 ACGCAATTAA TGTAAGTTAG CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA
1981 GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC
2041 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA
2101 TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT
2161 TCGGAATCTT GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG
2221 GCGAGAAGCA GGCCATTATC GCCGGCATGG CGGCCGACGC GCTGGGCTAC GTCTTGCTGG