pET-42b(+)图谱序列抗性价格Biovector质粒库

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pet42b载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列: 载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

EMD Biosciences(Novagen)

pET42b, pet 42b, pET-42b(+)

大肠杆菌蛋白表达

高

多克隆位点，限制性内切酶

5929bp

5'-TAATACGACTCACTATAGGG-3'

N-GST, N-His, C-His

Kanamycin

含有GST融合蛋白;N端Thrombin蛋白酶切位点; 含有N端Factor Xa蛋白酶切位点 ; pET 42 a,b,c 的差异仅在多克隆位点区域。

70562-3

瞬时表达 Transient

组成型 Constitutive

非病毒

pet42b载体质粒图谱和多克隆位点信息

pet42b载体图谱

Feature Name
Start
End

T7_terminator	120	1
T7_Terminal_primer	69	87
S15	350	306
6xHIS	410	393
GST (variant)	1091	441
T7_transl_en_RBS	1116	1100
lacO	1161	1134
T7_promoter	1179	1161
tet (300 - 563)	1215	1478
pBRrevBam_primer	1286	1267
lacI	1561	2652
ROP	3224	3415
pGEX_3_primer	3431	3409
pBR322_origin	4449	3830
KanR2	4555	5370

ORF
Start
End

ORF frame 3	1091	147
ORF frame 3	1092	1820
ORF frame 1	1693	2652
ORF frame 1	4555	5370

Enzyme Name
Cut

XhoI	174
NotI	182
EagI	182
HindIII	189
SalI	195
SacI	206
PstI	214
AscI	216
StuI	226
EcoRI	235
BamHI	241
EcoRV	251
NcoI	254
AgeI	288
KpnI	295
BglII	298
SacII	389
SpeI	420
MscI	883
NdeI	1090
XbaI	1128
ApaI	2131
HpaI	2426
NruI	4643
SmaI	4860
XmaI	4858

pet42b载体简介

The pET-42 series is designed for cloning and high-level expression of peptide sequencesfused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encodedsequence can be completely removed when cloning into the PshAI site (as shown below) and thencleaving the GST fusion protein with Factor Xa.

pet42b载体序列

LOCUS pET-42b(+) 5929 bp DNA circular SYN
DEFINITION pET-42b(+), pet42b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|Biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5929
/organism="pET-42 b(+)"
/mol_type="other DNA"
terminator complement(1..120)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(147..1091)
/label="ORF frame 3"
misc_feature complement(306..350)
/label="S15"
misc_feature complement(393..410)
/label="6xHIS"
gene complement(441..1091)
/label="GST (variant)"
/gene="GST (variant)"
CDS 1092..1820
/label="ORF frame 3"
misc_feature complement(1100..1116)
/label="T7_transl_en_RBS"
misc_feature complement(1134..1161)
/label="lacO"
promoter complement(1161..1179)
/label="T7_promoter"
gene 1215..1478
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(1267..1286)
/label="pBRrevBam_primer"
misc_feature 1561..2652
/label="lacI"
CDS 1693..2652
/label="ORF frame 1"
misc_feature 3224..3415
/label="ROP"
misc_feature complement(3409..3431)
/label="pGEX_3_primer"
rep_origin complement(3830..4449)
/label="pBR322_origin"
gene 4555..5370
/label="KanR2"
/gene="KanR2"
CDS 4555..5370
/label="ORF frame 1"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gac