pTrcHis2 C图谱序列抗性价格Biovector质粒库

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pTrcHis2C载体基本信息

出品公司:别名:质粒类型:表达水平:启动子:克隆方法:载体大小:5' 测序引物及序列:3' 测序引物及序列:载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

Invitrogen

pTrcHis2C, pTrcHis2 C

大肠杆菌蛋白表达载体

高拷贝

Trc

多克隆位点，限制性内切酶

4405 bp

pTrcHis-F: gaggtatatattaatgtatcg

pTrcHis-R:GATTTAATCTGTATCAGG

C-His, C-Myc

Ampicillin

Hosts: E.coli NM522, E.coli INValphaF'. Related vectors: pSE420.

V365-20

瞬时表达 Transient

组成型 Constitutive

非病毒

pTrcHis2C载体质粒图谱和多克隆位点信息

pTrcHis2C载体图谱

pTrcHis2C载体简介

The pTrcHis2 plasmids are pBR322-derived expression vectors designed for efficient recombinant protein expression and purification in E. coli. High levels of expression are possible using the trc (trp-lac) promoter (Egon et al., 1983) and the rrnB anti-termination region (Li et al., 1984). The trc promoter contains the –35 region of the trp promoter together with the –10 region of the lac promoter (Brosius et al., 1985; Egon et al., 1983; Mulligan et al., 1985). To regulate expression, the gene encoding Lac repressor (lacIq) is provided in the pTrcHis2 vectors, allowing regulation of the trc promoter regardless of whether the host strain contains a gene encoding the Lac repressor.

Isopropyl-Beta-D-thiogalactopyranoside (IPTG) is used to induce expression of your gene. Translation is enhanced by the bacteriophage T7 gene 10 translation enhancer and a minicistron that provides highly efficient translational restart into the open reading frame of the multiple cloning site. DNA inserts are positioned downstream and in frame with the initiation ATG and a C-terminal fusion peptide. The C-terminal peptide encodes the myc epitope and six histidine residues that function as a metal binding site in the expressed protein.

pTrcHis2C载体序列

LOCUS       pTrcHis2\C              4405 bp    DNA     circular SYN 7-JUL-2013
DEFINITION pTrcHis2 C.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     http://www.biofeng.com/
COMMENT     This file is created by Vector NTI
COMMENT     VNTOAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="pTrcHis2 A"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    235..257
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    264..333
                     /vntifkey="21"
                     /label=rrnB_ATS
     misc_feature    370..390
                     /vntifkey="21"
                     /label=pTrcHis_fwd_primer
     misc_feature    383..409
                     /vntifkey="21"
                     /label=minicis
     misc_feature    complement(588..605)
                     /vntifkey="21"
                     /label=pBAD_rev_primer
     misc_feature    complement(588..605)
                     /vntifkey="21"
                     /label=pTrcHis_rev_primer
     terminator      638..795
                     /vntifkey="43"
                     /label=rrnB_terminator
     terminator      761..804
                     /vntifkey="43"
                     /label=rrnB_T1_terminator
     terminator      936..963
                     /vntifkey="43"
                     /label=rrnB_T2_terminator
     promoter        1005..1033
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            1075..1935
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             1075..1935
                     /vntifkey="4"
                     /label=ORF\frame\2
     rep_origin      2090..2709
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    3106..3128
                     /vntifkey="21"
                     /label=pGEX_3_primer
     misc_feature    3275..4366
                     /vntifkey="21"
                     /label=lacI
     CDS             3407..4366
                     /vntifkey="4"
                     /label=ORF\frame\3
BASE COUNT     1092 a      1098 c      1161 g      1054 t
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
      301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
      361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatggatcc
      421 gagctcgaga tctgcagctg gtaccatatg ggaattcgaa gcttacgtag aacaaaaact
      481 catctcagaa gaggatctga atagcgccgt cgaccatcat catcatcatc attgagttta
      541 aacggtctcc agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt
      601 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
      661 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
      721 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
      841 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
      901 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
      961 ttttgcgttt ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg
     1021 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1081 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1141 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1201 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1261 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     1321 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1381 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1441 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1501 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1561 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1621 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1681 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1741 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1801 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1861 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1921 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1981 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2041 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2101 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2161 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2221 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2281 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2341 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2401 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2461 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2521 gaagggagaa aggcggacag gtatccggta agcggcaggg t