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pet11d载体基本信息
EMD Biosciences(Novagen) |
pET11d, pet 11d |
大肠杆菌蛋白表达 |
高 |
多克隆位点,限制性内切酶 |
5674bp |
T7 |
5'-TAATACGACTCACTATAGGG-3' |
N-T7 |
Ampicillin |
Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322. |
69439-3 |
瞬时表达 Transient |
组成型 Constitutive |
非病毒 |
pet11d载体质粒图谱和多克隆位点信息
T7_terminator | 316 | 188 |
T7_Terminal_primer | 256 | 274 |
T7_leader | 358 | 326 |
T7_transl_en_RBS | 382 | 366 |
lacO | 427 | 400 |
T7_promoter | 445 | 427 |
pBRrevBam_primer | 548 | 529 |
lacI | 823 | 1914 |
tet (576 - 1190) | 1973 | 2587 |
ROP | 3226 | 3417 |
pGEX_3_primer | 3433 | 3411 |
Ampicillin | 5466 | 4606 |
AmpR_promoter | 5536 | 5508 |
ORF frame 3 | 582 | 1082 |
ORF frame 1 | 955 | 1914 |
ORF frame 1 | 2392 | 1697 |
ORF frame 2 | 1970 | 2587 |
ORF frame 2 | 5466 | 4606 |
ClaI | 24 |
HindIII | 29 |
BamHI | 319 |
NheI | 350 |
NcoI | 355 |
XbaI | 394 |
BglII | 460 |
BclI | 1196 |
ApaI | 1393 |
HpaI | 1688 |
EagI | 2250 |
NruI | 2285 |
MscI | 2757 |
PstI | 4924 |
AatII | 5601 |
EcoRI | 5672 |
pet11d载体简介
The pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map. The cloning/expression region of the coding strand transcribed byT7 RNA polymerase is shown below.
pet11d载体序列
DEFINITION pET11d
ACCESSION
KEYWORDS
SOURCE
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