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载体基本信息
出品公司:
| Invitrogen |
载体名称:
| pcDNA3.1/Hygro(+) |
质粒类型:
| 哺乳动物(mammal;mammalian)细胞表达载体 |
启动子:
| CMV |
表达水平:
| 高 |
克隆方法:
| 多克隆位点，限制性内切酶 |
载体大小:
| 5597 bp |
5' 测序引物:
| pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
3' 测序引物:
| pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
载体标签:
| C-His, C-Myc |
载体抗性:
| Ampicillin 氨苄 |
筛选标记:
| Hygromycin 潮霉素 |
备注:
Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori. |
产品目录号:
| V870-20 |
稳定性:
| 瞬表达 Transient |
组成型:
| 组成型 Constitutive |
病毒/非病毒:
| 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
pcDNA3.1/Hygro (+) and pcDNA3.1/Hygro (–) are 5.6 kb vectors derived from pcDNA3.1 and are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. These vectors contain the following elements:
•Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
•Multiple cloning sites in the forward (+) and reverse (–) orientations to facilitate cloning
•Hygromycin resistance gene for selection of stable cell lines
•Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS–1, COS–7)
A control plasmid, pcDNA3.1/Hygro/lacZ, is included for use as a positive control for transfection and expression in the cell line of choice.
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