BL21 Star(DE3)菌株感受态细胞抗性价格报价Biovector菌株库现货

nce restriction site

Description

E. coli BL21 Star (DE3) is designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., pET vectors).

• Contain a genotype that promotes high mRNA stability and protein yield
• Optimized for use with low copy number, T7 promoter-based plasmids
• Provided in a single-tube, single-use format—allows all transformation steps to take place in the same tube, thereby helping to save time and prevent contamination

Enhanced expression of nontoxic recombinant proteins
BL21 Star (DE3) cells contain the DE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promoter. IPTG is required to induce expression. This strainalso offers enhanced mRNA stability due to a mutation in the RNaseE gene (rne131) that reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield. Protein expression is further enhanced by the absence of thelon and outer membrane (OmpT) proteases, which reduces degradation of heterologous proteins.

Note: The BL21 Star strains have higher basal expression of heterologous genes than BL21 strains, due to the increased stability of mRNA. Therefore, these strains may not be useful for expression of toxic genes.

Genotype:F^-ompT hsdS_B (r_B^-, m_B^-) galdcmrne131 (DE3)

 

BL21(DE3)Star感受态细胞基本信息

出品公司:感受态 细胞名称:菌株类型:产品目录号:培养基:生长条件:基因型:抗性:应用:菌株特点:

Invitrogen

BL21 Star(DE3), BL21(DE3)Star

E.coli

C6010-03

LB培养基

37 ℃, 有氧

无

蛋白表达

来源于BL21(DE3)菌株。 含有rne131基因突变体，rne131突变基因能够增强菌株细胞内mRNA的稳定性，从而有效提高蛋白表达能力。 主要适用于T7启动子载体的高水平蛋白表达。

BL21(DE3)Star感受态细胞说明书pdf版和相关资料下载

BL21(DE3)Star感受态细胞应用举例